Sam's Notebook » hybridization http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Hibridizaton/Ligation SOLiD Libraries – Abalone, Yellow Perch, Lake Trout, Herring http://onsnetwork.org/kubu4/2010/04/05/hibridizatonligation-solid-libraries-abalone-yellow-perch-lake-trout-herring/ http://onsnetwork.org/kubu4/2010/04/05/hibridizatonligation-solid-libraries-abalone-yellow-perch-lake-trout-herring/#comments Mon, 05 Apr 2010 17:30:24 +0000 http://onsnetwork.org/kubu4/?p=680

All 8 samples were hybridized/ligated according to Ambion’s Whole Transcriptome Analysis Kit using Adaptor A.

]]> http://onsnetwork.org/kubu4/2010/04/05/hibridizatonligation-solid-libraries-abalone-yellow-perch-lake-trout-herring/feed/ 0 RNA Adapter Hybridization and Ligation – Herring Liver mRNA for SOLiD Libraries http://onsnetwork.org/kubu4/2009/12/08/rna-adapter-hybridization-and-ligation-herring-liver-mrna-for-solid-libraries/ http://onsnetwork.org/kubu4/2009/12/08/rna-adapter-hybridization-and-ligation-herring-liver-mrna-for-solid-libraries/#comments Wed, 09 Dec 2009 00:34:55 +0000 http://onsnetwork.org/kubu4/?p=762

RNA from yesterday was speedvac’d to dryness and resuspended in 3uL of nuclease-free H2O. Samples were mixed with Adaptor Mix A and hybridized according to Ambion WTK protocol. Samples were then ligated for 16hrs @ 16C, according to Ambion WTK protocol.

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