Sam's Notebook » ladder http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Reagent Prep – RNA Pico 6000 Ladder http://onsnetwork.org/kubu4/2015/12/18/reagent-prep-rna-pico-6000-ladder/ http://onsnetwork.org/kubu4/2015/12/18/reagent-prep-rna-pico-6000-ladder/#comments Sat, 19 Dec 2015 04:33:47 +0000 http://onsnetwork.org/kubu4/?p=1902

Prepared the RNA Pico 6000 ladder (Agilent) according to the manufacturer’s protocol:

  • Denatured @ 70C for 2mins
  • Immediately put on ice
  • Added 90μL H2O

Made 2μL aliquots of the ladder in 0.5mL snap cap tubes. Samples were boxed, labeled, and stored @ -80C (Rack #9).

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