Sam's Notebook » Martha’s Vineyard http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 MBL Shipment – MV oysters/cod http://onsnetwork.org/kubu4/2009/11/19/mbl-shipment-mv-oysterscod/ http://onsnetwork.org/kubu4/2009/11/19/mbl-shipment-mv-oysterscod/#comments Fri, 20 Nov 2009 01:21:37 +0000 http://onsnetwork.org/kubu4/?p=788

Received a shipment of various MV oysters/cod samples from Scott Lindell at MBL. However, these were NOT shipped on dry ice! Samples were put @ -80C. Will be organized at a later date.

]]> http://onsnetwork.org/kubu4/2009/11/19/mbl-shipment-mv-oysterscod/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/07/qpcr-mv-hemocyte-cdna-from-20090614/ http://onsnetwork.org/kubu4/2009/07/07/qpcr-mv-hemocyte-cdna-from-20090614/#comments Tue, 07 Jul 2009 22:32:52 +0000 http://onsnetwork.org/kubu4/?p=965

Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:

3326A13

2100B07

3219A06

2100B15

3326A11

2100B12

2100A03

Plate layout/PCR set up is here. This is a second rep of these samples.

Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:

]]> http://onsnetwork.org/kubu4/2009/07/07/qpcr-mv-hemocyte-cdna-from-20090614/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/06/qpcr-mv-hemocyte-cdna-from-20090614-2/ http://onsnetwork.org/kubu4/2009/07/06/qpcr-mv-hemocyte-cdna-from-20090614-2/#comments Mon, 06 Jul 2009 22:39:30 +0000 http://onsnetwork.org/kubu4/?p=973

Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:

3326A13

2100B07

3219A06

2100B15

3326A11

2100B12

2100A03

Plate layout/PCR set up is here.

Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:

]]> http://onsnetwork.org/kubu4/2009/07/06/qpcr-mv-hemocyte-cdna-from-20090614-2/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/02/qpcr-mv-hemocyte-cdna-from-20090614-3/ http://onsnetwork.org/kubu4/2009/07/02/qpcr-mv-hemocyte-cdna-from-20090614-3/#comments Thu, 02 Jul 2009 22:44:42 +0000 http://onsnetwork.org/kubu4/?p=977

Set up qPCR with Cv_CIAPIN_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/07/02/qpcr-mv-hemocyte-cdna-from-20090614-3/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-6/ http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-6/#comments Wed, 01 Jul 2009 22:55:44 +0000 http://onsnetwork.org/kubu4/?p=983

Set up qPCR with Cv_CatY_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-6/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-5/ http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-5/#comments Wed, 01 Jul 2009 22:53:25 +0000 http://onsnetwork.org/kubu4/?p=981

Set up qPCR with Cv_CatL_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-5/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-4/ http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-4/#comments Wed, 01 Jul 2009 22:51:16 +0000 http://onsnetwork.org/kubu4/?p=979

Set up qPCR with Cv_TLR_”short”_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/07/01/qpcr-mv-hemocyte-cdna-from-20090614-4/feed/ 0 qPCRs – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/06/30/qpcrs-mv-hemocyte-cdna-from-20090614/ http://onsnetwork.org/kubu4/2009/06/30/qpcrs-mv-hemocyte-cdna-from-20090614/#comments Tue, 30 Jun 2009 22:58:25 +0000 http://onsnetwork.org/kubu4/?p=985

qPCR – BgBL Primers

Set up qPCR with Cv_BgBL_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR – HMG Primers

Set up qPCR with Cv_HMG_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR – HSP70 Primers

Set up qPCR with Cv_HSP70_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/06/30/qpcrs-mv-hemocyte-cdna-from-20090614/feed/ 0 qPCRs – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/06/29/qpcrs-mv-hemocyte-cdna-from-20090614-2/ http://onsnetwork.org/kubu4/2009/06/29/qpcrs-mv-hemocyte-cdna-from-20090614-2/#comments Mon, 29 Jun 2009 23:01:26 +0000 http://onsnetwork.org/kubu4/?p=987

qPCR – CysB Primers

Set up qPCR with Cv_CysB_F/R. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR -BgBp Primers

Set up qPCR with Cv_BgBp_F/R primers. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/06/29/qpcrs-mv-hemocyte-cdna-from-20090614-2/feed/ 0 qPCR – MV hemocyte cDNA from 20090614 http://onsnetwork.org/kubu4/2009/06/26/qpcr-mv-hemocyte-cdna-from-20090614-7/ http://onsnetwork.org/kubu4/2009/06/26/qpcr-mv-hemocyte-cdna-from-20090614-7/#comments Fri, 26 Jun 2009 23:03:49 +0000 http://onsnetwork.org/kubu4/?p=989

Set up qPCR with Cv_18s_F/R primers. Plate layout/PCR set up is here. This is a second rep. Used 1:20 cDNA plate made by Mac as template.

Results: Waters are clean. Still some samples that aren’t coming up. Will eventually run those samples using undiluted cDNA. To be analyzed later with other genes that Mac has run.

]]> http://onsnetwork.org/kubu4/2009/06/26/qpcr-mv-hemocyte-cdna-from-20090614-7/feed/ 0