Sam's Notebook » oligos http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Oligo Reconstitution – Illumina RNAseq Library Oligos and Barcodes http://onsnetwork.org/kubu4/2012/07/11/oligo-reconstitution-illumina-rnaseq-library-oligos-and-barcodes/ http://onsnetwork.org/kubu4/2012/07/11/oligo-reconstitution-illumina-rnaseq-library-oligos-and-barcodes/#comments Wed, 11 Jul 2012 23:25:59 +0000 http://onsnetwork.org/kubu4/?p=231

Reconstituted all of the oligos and barcodes for library construction in TE (pH = 8.0) to a final concentration of 100uM. Created 10uM working stocks of all oligos and barcodes. All samples (stocks and working stocks) are stored @ -80C in their own box (Illumina Library Oligos & Barcodes) due to the fact that one of the oligos is an RNA oligo and requires storage at -80C.

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