Sam's Notebook » plasmid isolation http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Minipreps – Emma’s Illumina Library Cloning http://onsnetwork.org/kubu4/2012/07/23/minipreps-emmas-illumina-library-cloning/ http://onsnetwork.org/kubu4/2012/07/23/minipreps-emmas-illumina-library-cloning/#comments Mon, 23 Jul 2012 23:23:35 +0000 http://onsnetwork.org/kubu4/?p=229

Performed plasmid isolation on 17 bacterial cultures Emma inoculated yesterday using the QIAprep Spin Mini Kit (Qiagen) using ~1.4mL of culture according to the manufacturer’s protocol. Plasmid DNA was eluted with 50uL of Buffer EB. Tubes were stored @ 4C in the refrigerator in FTR 213.

]]> http://onsnetwork.org/kubu4/2012/07/23/minipreps-emmas-illumina-library-cloning/feed/ 0 Mini-preps – COX/PGS Cloning Colonies from today http://onsnetwork.org/kubu4/2011/10/13/mini-preps-coxpgs-cloning-colonies-from-today/ http://onsnetwork.org/kubu4/2011/10/13/mini-preps-coxpgs-cloning-colonies-from-today/#comments Fri, 14 Oct 2011 02:13:38 +0000 http://onsnetwork.org/kubu4/?p=292

Selected 10 colonies (1-8, 18, 28) for mini-preps. Inoculated 5mL 1x LB + 50ug/mL of Kanamycin. Incubated O/N, 37C, 200RPM. 3mL of each culture were used for mini-preps. Used Qiagen kit. Samples were eluted w/30uL of EB.

]]> http://onsnetwork.org/kubu4/2011/10/13/mini-preps-coxpgs-cloning-colonies-from-today/feed/ 0 Plasmid Isolation – Miniprep on PGS Hi 4 Colony from yesterday http://onsnetwork.org/kubu4/2011/07/12/plasmid-isolation-miniprep-on-pgs-hi-4-colony-from-yesterday/ http://onsnetwork.org/kubu4/2011/07/12/plasmid-isolation-miniprep-on-pgs-hi-4-colony-from-yesterday/#comments Wed, 13 Jul 2011 03:26:10 +0000 http://onsnetwork.org/kubu4/?p=331

Plasmid was isolated from 3mL of liquid culture started yesterday using the Qiagen MiniPrep Kit, according to the manufacturer’s protocol. Will send off for sequencing.

]]> http://onsnetwork.org/kubu4/2011/07/12/plasmid-isolation-miniprep-on-pgs-hi-4-colony-from-yesterday/feed/ 0 Mini Preps – Liquid Cultures from yesterday http://onsnetwork.org/kubu4/2011/04/26/mini-preps-liquid-cultures-from-yesterday/ http://onsnetwork.org/kubu4/2011/04/26/mini-preps-liquid-cultures-from-yesterday/#comments Tue, 26 Apr 2011 20:44:34 +0000 http://onsnetwork.org/kubu4/?p=372

Mini prepped 3mLs of each culture, according to Qiagen protocol. Samples were eluted with 50uL of Buffer EB.

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