Sam's Notebook » PRJNA316624 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Data Management – SRA Submission Olympia Oyster UW PacBio Data from 20170323 http://onsnetwork.org/kubu4/2017/07/06/data-management-sra-submission-olympia-oyster-uw-pacbio-data-from-20170323/ http://onsnetwork.org/kubu4/2017/07/06/data-management-sra-submission-olympia-oyster-uw-pacbio-data-from-20170323/#comments Thu, 06 Jul 2017 22:47:54 +0000 http://onsnetwork.org/kubu4/?p=2633

Submitted the FASTQ files from the UW PacBio Data from 20170323 to the NCBI sequence read archive (SRA).

FTP’d the data to NCBI’s servers, following their instructions. Briefly,

Change to the directory where the FASTQ files are (Owl/web/nightingales/O_lurida) and then initiate an FTP session:

ftp -i ftp-private.ncbi.nlm.nih.gov

Enter provided username/password, change to my designated uploads directory, create new folder dedicate to this particular upload. Then, upload all the files using the mput command:

mput *filtered_subreads*

SRA deets are below (assigned FASTQ files to existing BioProject and created a new BioSample). Will update post with SRA number when processing is complete on the NCBI end.

SRA: SRS2339870
Study: SRR5809355
BioProject: PRJNA316624
BioSample: SAMN07326085

]]>
http://onsnetwork.org/kubu4/2017/07/06/data-management-sra-submission-olympia-oyster-uw-pacbio-data-from-20170323/feed/ 0
SRA Submission – Genome sequencing of the Olympia oyster (Ostrea lurida) http://onsnetwork.org/kubu4/2016/03/28/sra-submission-genome-sequencing-of-the-olympia-oyster-ostrea-lurida/ http://onsnetwork.org/kubu4/2016/03/28/sra-submission-genome-sequencing-of-the-olympia-oyster-ostrea-lurida/#comments Mon, 28 Mar 2016 23:21:12 +0000 http://onsnetwork.org/kubu4/?p=2066

Adding our Olympia oyster genome sequencing (sequencing done by BGI) to the NCBI Sequence Read Archive (SRS). The current status can be seen in the screen cap below. Release date is set for a year from now, but will likely bump it up. Need Steven to review the details of the submission (BioProject, Experiment descriptions, etc.) before I initiate the public release. Will update this post with the SRA number once we receive it.

Here’s the list of files uploaded to the SRA:

151114_I191_FCH3Y35BCXX_L1_wHAIPI023992-37_1.fq.gz
151114_I191_FCH3Y35BCXX_L1_wHAIPI023992-37_2.fq.gz
151114_I191_FCH3Y35BCXX_L2_wHAMPI023991-66_1.fq.gz
151114_I191_FCH3Y35BCXX_L2_wHAMPI023991-66_2.fq.gz
151118_I137_FCH3KNJBBXX_L5_wHAXPI023905-96_1.fq.gz
151118_I137_FCH3KNJBBXX_L5_wHAXPI023905-96_2.fq.gz
160103_I137_FCH3V5YBBXX_L3_WHOSTibkDCABDLAAPEI-62_1.fq.gz
160103_I137_FCH3V5YBBXX_L3_WHOSTibkDCABDLAAPEI-62_2.fq.gz
160103_I137_FCH3V5YBBXX_L3_WHOSTibkDCACDTAAPEI-75_1.fq.gz
160103_I137_FCH3V5YBBXX_L3_WHOSTibkDCACDTAAPEI-75_2.fq.gz
160103_I137_FCH3V5YBBXX_L4_WHOSTibkDCABDLAAPEI-62_1.fq.gz
160103_I137_FCH3V5YBBXX_L4_WHOSTibkDCABDLAAPEI-62_2.fq.gz
160103_I137_FCH3V5YBBXX_L4_WHOSTibkDCACDTAAPEI-75_1.fq.gz
160103_I137_FCH3V5YBBXX_L4_WHOSTibkDCACDTAAPEI-75_2.fq.gz
160103_I137_FCH3V5YBBXX_L5_WHOSTibkDCAADWAAPEI-74_1.fq.gz
160103_I137_FCH3V5YBBXX_L5_WHOSTibkDCAADWAAPEI-74_2.fq.gz
160103_I137_FCH3V5YBBXX_L6_WHOSTibkDCAADWAAPEI-74_1.fq.gz
160103_I137_FCH3V5YBBXX_L6_WHOSTibkDCAADWAAPEI-74_2.fq.gz

Paired-end sequencing files were uploaded together within a single “Run”.

SRA Info:
SRA: SRS1365663
Study: SRP072461
BioProject: PRJNA316624
BioSample: SAMN04588827

]]>
http://onsnetwork.org/kubu4/2016/03/28/sra-submission-genome-sequencing-of-the-olympia-oyster-ostrea-lurida/feed/ 0