Sam's Notebook » QIAQuick Gel Extraction Kit http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Gel Extraction – Oly RAD-Seq Prep Scale PCR http://onsnetwork.org/kubu4/2015/11/16/gel-extraction-oly-rad-seq-prep-scale-pcr/ http://onsnetwork.org/kubu4/2015/11/16/gel-extraction-oly-rad-seq-prep-scale-pcr/#comments Tue, 17 Nov 2015 03:47:10 +0000 http://onsnetwork.org/kubu4/?p=1782

Extracted the PCR products from the gel slices from 20151113 using the QIAQuick Gel Extraction Kit (Qiagen) according to the manufacturer’s protocol. Substituted MiniElute columns so that I could elute with a smaller volume than what is used in the QIAQuick standard protocol.

Samples were eluted with 20μL of Buffer EB.

Will quantify these libraries via qPCR.

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