Sam's Notebook » QPX challenge http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Hard Clam Challenge – QPX Strain S-1 (continued from yesterday) http://onsnetwork.org/kubu4/2009/12/02/hard-clam-challenge-qpx-strain-s-1-continued-from-yesterday/ http://onsnetwork.org/kubu4/2009/12/02/hard-clam-challenge-qpx-strain-s-1-continued-from-yesterday/#comments Thu, 03 Dec 2009 01:05:24 +0000 http://onsnetwork.org/kubu4/?p=776

All clams appeared to be alive and well. Most had their siphons out when I arrived to start collecting tissues. Clams were shucked after 24hr challenge. Gill and mantle samples were collected in separate 1.5mL snap cap tubes, stored briefly on ice and transferred to -80C in “Hard Clam QPX Challenge 12/2/2009.” box.

]]> http://onsnetwork.org/kubu4/2009/12/02/hard-clam-challenge-qpx-strain-s-1-continued-from-yesterday/feed/ 0 Hard Clam Challenge – QPX Strain S-1 http://onsnetwork.org/kubu4/2009/12/01/hard-clam-challenge-qpx-strain-s-1/ http://onsnetwork.org/kubu4/2009/12/01/hard-clam-challenge-qpx-strain-s-1/#comments Wed, 02 Dec 2009 01:11:38 +0000 http://onsnetwork.org/kubu4/?p=780

Challenged 2 FL hard clams and 1 BX hard clam with ~100uL of unwashed, 11 day old cultures. 2 FL hard clams and 1 BX hard clam received ~100uL of QPX media, as controls. Injections were done through the hinge using 20G1 needles and aimed for the pericardial cavity. After injections, clams were left out of water for 1.5hrs, then return to small containers of sea water. They will be incubated for 24hrs.

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