Hollie Putnam prepared some reduced representation bisulfite Illumina libraries and had them sequenced by Genewiz.
The data was downloaded and MD5 checksums were generated.
IMPORTANT: MD5 checksums have not yet been provided by Genewiz! We cannot verify the integrity of these data files at this time! Checksums have been requested. Will create new notebook entry (and add link to said entry) once the checksums have been received and we can compare them.
She needed these samples combined in to four separate pools. However, Pool 5 was to be pooled with a total of five samples, including EPI_135 WG. She asked that the EPI_135 WG sample make up 50% of the DNA in the pool.
Using her previously determined sample concentrations, I pooled the libraries in equal quantities.
The pool volumes are high and, the calculated pool concentrations are low. Due to time limitations on our end, it was not feasible for me to SpeedVac these down to achieve the target concentration of 10nM. I’ve notified Hollie and asked her to see if Genewiz will perform that service.
Samples were shipped on dry ice to Genewiz via FedEx Standard Overnight.