Sam's Notebook » SpeedVac http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 DNA Concentration – Acropora cervicornis (Staghorn coral) DNA from Javier Casariego (FIU) http://onsnetwork.org/kubu4/2017/05/10/dna-concentration-acropora-cervicornis-staghorn-coral-dna-from-javier-casariego-fiu/ http://onsnetwork.org/kubu4/2017/05/10/dna-concentration-acropora-cervicornis-staghorn-coral-dna-from-javier-casariego-fiu/#comments Wed, 10 May 2017 22:22:17 +0000 http://onsnetwork.org/kubu4/?p=2573

Three samples (of the 62 total) that were quantified earlier today, had concentrations too low for use in the methylation assay:

  • 2h Block 1
  • 2h Block 8
  • D35 Block 8

These samples were dried to completion in a SpeedVac.

They will be allowed to rehydrate O/N in 10uL of Buffer EB (Qiagen) and will be re-quantified tomorrow morning.

]]> http://onsnetwork.org/kubu4/2017/05/10/dna-concentration-acropora-cervicornis-staghorn-coral-dna-from-javier-casariego-fiu/feed/ 1 SpeedVac – C.gigas larvae OA DNA http://onsnetwork.org/kubu4/2015/01/12/speedvac-c-gigas-larvae-oa-dna/ http://onsnetwork.org/kubu4/2015/01/12/speedvac-c-gigas-larvae-oa-dna/#comments Tue, 13 Jan 2015 00:12:01 +0000 http://onsnetwork.org/kubu4/?p=27

The DNA extracted and sheared on 20150109 was in a volume too great to proceed with bisulfite conversion.  Dried the samples to complete dryness in a SpeedVac (~4hrs).  Reconsitituted DNA in 24μL of NanoPure water.  Will bisulfite convert tomorrow.

Sample list:

SAMPLE ID DATE TREATMENT (ppm) # LARVAE TOTAL DNA (ng)
6B5 20110513 400 5,000 105
1B2 20110513 1000 5,000 109
6B2 20110513 400 10,000 117
1B1 20110513 1000 10,000 593
1B1 20110519 1000 NA 500
1B2 20110519 1000 NA 500
6B2 20110519 400 NA 269
6B1 20110519 400 NA 345

 

Updated the quantification spreadsheet with a column labeled “[New](ug/uL)” that calculates the new concentrations of the above samples in the 24μL volume.

https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing

 

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