The DNA extracted and sheared on 20150109 was in a volume too great to proceed with bisulfite conversion. Dried the samples to complete dryness in a SpeedVac (~4hrs). Reconsitituted DNA in 24μL of NanoPure water. Will bisulfite convert tomorrow.
Sample list:
SAMPLE ID |
DATE |
TREATMENT (ppm) |
# LARVAE |
TOTAL DNA (ng) |
6B5 |
20110513 |
400 |
5,000 |
105 |
1B2 |
20110513 |
1000 |
5,000 |
109 |
6B2 |
20110513 |
400 |
10,000 |
117 |
1B1 |
20110513 |
1000 |
10,000 |
593 |
1B1 |
20110519 |
1000 |
NA |
500 |
1B2 |
20110519 |
1000 |
NA |
500 |
6B2 |
20110519 |
400 |
NA |
269 |
6B1 |
20110519 |
400 |
NA |
345 |
Updated the quantification spreadsheet with a column labeled “[New](ug/uL)” that calculates the new concentrations of the above samples in the 24μL volume.
https://docs.google.com/spreadsheets/d/1e7EF05akWeBtO7Xz0UWXhIzRlkVU5HA_rjCE3c4SPEw/edit?usp=sharing