Repeat of PCR from 20090403, but using AmpliTaq and 50C annealing temp. PCR set up is here.

Lane 1 - Hyperladder

Lane 2 - 19t

Lane 3 - 100bp ladder

Lane 4 - 13t

Lane 6 - 17t

Lane 8 - 1.5t

Lane 9 - Hyperladder

Lane 10 - 1.2t

Lane 12 - 11t

Lane 13 - 100bp ladder

Lane 14 - H5

Lane 16 - 12t

Lane 17 - 100bp Ladder

Lane 18 - H2O

Lane 19 - H2O

Lane 20 - Hyperladder

Results: Nothing amplified! Possibly due to age of polymerase (?); over a year old. Will wait to repeat for new primers to arrive (EukA/B).