Used 3 sets of reverse primers:

Bay_Actin_Rv0

Bay_Actin_Rv2

Sea_Actin_Rv2

Primers were slected based on information from Steven’s notebook (#8, 12/30/2007-1/3/2008). Anneal temp 53C.

PCR set up here . Plate layout here .

Samples were run out by Steven the following day.

Gel 1 of 3

Gel 2 of 3

Gel 3 of 3

Results: