Lane 1 - 15ng standard (5uL)

Lane 2 - 31ng standard (5uL)

Lane 3 - 63ng standard (5uL)

Lane 4 - Marker 2 (5uL)

Lane 5 - C. pugetti DNA (5uL: 4uL + 1uL 5x dye)

Lane 6 - Marker 3 (5uL)

Lane 7 - 125ng standard

Lane 8 - 250ng standard (5uL)

Lane 9 - 500ng standard (5uL)

Results: Looks great! Will run PCR using universal 16s primers for sequencing.