qPCR was set up on these cDNAs using the following primers:
EW778094 (“Ficolin 3”) -
AJ422120.p.cg.6 (“MDR49”, “Multidrug resistance protein homolog 49”) - This was upregulated in DH SOLiD data.
qPCR set up and plate layout can be found here.
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Results:
qPCR Data File (Opticon): 20091224_092959.tad
Data workup: PROPS BB_DH Gene Expression Miner Workup
Preliminary results show that the EW778094 primer set looks bad; bad fluorescence profile and poor melt curves. Primers may require optimization.
qPCR was set up on these cDNAs using the following primers:
AM855874.p.cg.6 (“CP17A”, “Steroid 17-alpha-hydroxylase/17”) - This was upregulated in DH SOLiD data.
AM857078.p.cg.6 (“C1QT4”, Complement C1q tumor necrosis factor-related protein 4”) - This was upregulated in DH SOLiD data.
qPCR set up and plate layout can be found here.
Results:
qPCR Data File (Opticon): 20091224_125230.tad
Data workup: PROPS BB_DH Gene Expression Miner Workup