Ran colony PCR on various colonies produced from cloning on 20110411. All colonies were picked, re-streaked on Kan50 plate(s) and PCR’d. Master mix calcs are here. Cycling params:

• 95C - 10mins

40cycles of:

• 95C - 30s

• 55C - 30s

• 72C - 3mins

• 72C - 10mins

Results: