Precipitation was continued according to the MethylMiner Methylated DNA Enrichment Kit (Invitrogen). Since I will need sample volumes of 24uL for the subsequent bisulfite conversion, I resuspended the samples in 29uL of water (will use 2.5uL x 2 reps for quantification).
Samples to be quantified:
NC = non-captured (i.e. non-methylated)
E = eluted (i.e. methylated)
-
HB2 NC
-
HB5 NC
-
HB16 NC
-
HB30 NC
-
NB3 NC
-
NB6 NC
-
NB11 NC
-
NB21 NC
-
HB2 E
-
HB5 E
-
HB16 E
-
HB30 E
-
NB3 E
-
NB6 E
-
NB11 E
-
NB21 E
-
Control NC
-
Control E
Samples were quantified using the Quant-IT BS Kit (Invitrogen) with a plate reader (BioTek). All samples were run in duplicate. Used 2.5uL of each sample for quantification.
Samples were stored in @ -20C (FTR 209) in the bisulfite seq box created by Claire for this project.
Results:
20141202_LSU_Virginica_MBD:
https://docs.google.com/spreadsheets/d/1NrrVmYsUQcstnrt4583mYN2PeVav54luyFvVUEkcjWE/edit?usp=sharing