Continued library prep from yesterday. Set up Library Amplification according to the protocol. The samples received the following Barcode Indices:
-
HB2 - 1 (ATCACG)
-
HB5 - 2 (CGATGT)
-
HB16 - 3 (TTAGGC)
-
HB30 - 4 (TGACCA)
-
NB3 - 5 (ACAGTG)
-
NB6 - 6 (GCCAAT)
-
NB11 - 7 (CAGATC)
-
NB21 - 12 (CTTGTA)
-
1A1 - 2 (CGATGT)
-
1A2 - 1 (ATCACG)
-
6A1 - 4 (TGACCA)
-
6A2 - 5 (ACAGTG)
-
103B1 - 6 (GCCAAT)
-
103B2 - 7 (CAGATC)
-
105A4 - 12 (CTTGTA)
-
105A5 - 11 (GGCTAC)
Due to differences in input DNA quantities, samples were run with different numbers of thermal cycles.
13 thermal cycles were run for the following samples:
-
1A1
-
105A4
-
105A5
22 thermal cycles were run for the following samples:
-
HB2
-
HB5
-
HB16
-
HB30
-
NB3
-
NB6
-
NB11
-
NB21
-
1A2
-
6A1
-
6A2
-
103B1
-
103B2
Samples were quantified with 1uL of each sample using the Quant-iT dsDNA BR Kit (Invitrogen). Used 5uL of each standard and standards were run in duplicate.
Results: