Ran qPCR on DNased RNA from earlier today to assess whether there was any residual gDNA after the DNase treatment with Oly_Actin_F/R primers (SR IDs: 1505, 1504).

Used 1μL from all templates.

All samples were run in duplicate.

Positive control was HL1 O.lurida DNA isolated by Jake on 20150323.

Cycling params:

• 95C – 2.5mins

• 40 cycles of:

  • 95C – 10s

  • 60C – 20s

• Melt curve

Master mix calcs are here (used same calcs from the other day): 20150512_qPCR_Oly_RNA

Plate layout: 20150514_qPCR_plate_Jake_Oly_1hr_HS_DNased_RNA

Results:

qPCR Data File (Opticon): Sam_20150514_170332.tad

qPCR Report (Google Spreadsheeet): 20150514_qPCR_Report_Jake_Oly_DNased_1hr_HS_RNA

Positive control samples are the only samples that produced amplification (cycle ~20). Will proceed to making cDNA.

Amplification Plots

(http://eagle.fish.washington.edu/Arabidopsis/20150514_qPCR_Amp_DNased_RNA_Jake_Oly_1hr_HS.JPG)

Melt Curves

(http://eagle.fish.washington.edu/Arabidopsis/20150514_qPCR_Melt_DNased_RNA_Jake_Oly_1hr_HS.JPG)