Isolated gDNA from Panopea generosa (geoduck) adductor muscle & foot samples collected by Brent & Steven on 20150811 using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek) according to the manufacturer’s protocol, with the following adjustments:

• 41.8mg of adductor muscle

• 30.0mg of foot used

• Tissues homogenized in 350μL of ML1 Buffer with disposable mortar/pestle tubes using only three pestles strokes

• Homogenized tissue incubated in ML1 Buffer + Proteinase K @ 60C for 2.5hrs

• Added 265μL of MBL Buffer

• Added 514μL of 100% EtOH.

• Eluted with 75μL Elution Buffer.

Spec’d on Roberts Lab NanoDrop1000 (ThermoFisher) and stored temporarily at 4C to avoid freeze-thawing before sending off for sequencing next week.

Results:

(http://eagle.fish.washington.edu/Arabidopsis/20150828_geoduck_gDNA_ODs.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150828_geoduck_gDNA_plots.JPG)

Yields are good (~7ug).

Quality (260/280 ratios) looks great for both samples.

260/230 ratio not very good for adductor muscle, but perfect for foot tissue.

Will run samples on gel to assess gDNA integrity.