Continued the extractions that Steven began yesterday and this morning using the E.Z.N.A. Mollusc DNA Kit (Omega Bio-Tek) after the RNase treatment @ 70C.

• Samples were cooled to RT (~10mins)

• Added 300μL of 100% EtOH to each (equivalent to the volume of aqueous phase Steven recovered from each sample)

• Followed manufacturer’s protocol

• Eluted all samples with 50μL of Elution Buffer

• Spec’d on Roberts Lab NanoDrop1000

Results:

(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_ODs_01.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_ODs_02.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_plots_01.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_plots_02.JPG)

DNA looks absolutely pristine and has amazing yields.

Will check gDNA integrity via agarose gel tomorrow.

Data has been entered into the master spreadsheet for this project: Ostrea lurida Project Master Tissue Sample List