Pooled the gDNA samples from earlier today & from 20151002.

The pooled volume = 260μL

Quantified on the Roberts Lab NanoDrop1000.

Results:

(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_ODs.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_plots.JPG)

Besides the weird peak at 240nm, everything looks great - perfect 260/280 & 260/230 ratios.

Yield = 75.92μg

This gDNA has been RNase treated, so the concentration should be semi-accurate. Regardless, even if there’s a discrepancy of 50%, this should provide enough additional DNA for BGI to work with.

Will run on gel to evaluate integrity and then send to BGI.