We opted to go with ZymoResearch for this project because they could do the bisulfite treatment and finish the sequencing by the end of January.

Submitted the following 18 Ostrea lurida MBD-enriched gDNA samples to ZymoResearch for bisulfite treatment and subsequent Illumina sequencing (50bp, single read):

hc1_2B

hc1_4B

hc2_15B

hc2_17

hc3_1

hc3_10

hc3_11

hc3_5

hc3_7

hc3_9

ss2_14B

ss2_18B

ss2_9B

ss3_14B

ss3_15B

ss3_16B

ss3_20

ss3_3B

ss3_4B

ss5_18

The samples will be bisulfite treated, Illumina libraries constructed, multiplexed, and this multiplexed library will be sequenced three times.