Re-quantified the libraries that were completed yesterday using the Qubit3.0 dsDNA HS (high sensitivity) assay because the library concentrations were too low for the normal broad range kit.

Results:

Qubit Quants and Library Normalization Calcs: 20151222_qubit_illumina_methylation_libraries

<td data-sheets-value="[null,2,"Test Name"]" >**SAMPLE** </td> <td data-sheets-value="[null,2,"Original sample conc."]" >**CONCENTRATION (ng/μL)** </td> <td data-sheets-value="[null,2,"1NF11"]" >1NF11 </td> <td data-sheets-value="[null,2,"1NF15"]" >1NF15 </td> <td data-sheets-value="[null,2,"1NF16"]" >1NF16 </td> <td data-sheets-value="[null,2,"1NF17"]" >1NF17 </td> <td data-sheets-value="[null,2,"2NF5"]" >2NF5 </td> <td data-sheets-value="[null,2,"2NF6"]" >2NF6 </td> <td data-sheets-value="[null,2,"2NF7"]" >2NF7 </td> <td data-sheets-value="[null,2,"2NF8"]" >2NF8 </td> <td data-sheets-value="[null,2,"M2"]" >M2 </td> <td data-sheets-value="[null,2,"M3"]" >M3 </td> <td data-sheets-value="[null,2,"NF2_6"]" >NF2_6 </td> <td data-sheets-value="[null,2,"NF_18"]" >NF_18 </td>

2.42
1.88
2.74
2.54
2.72
2.44
2.38
1.88
2.18
2.56
2.5
2.66

Things look pretty good. The [TruSeq DNA Methylation Library Kit (Illumina)(https://github.com/sr320/LabDocs/blob/master/protocols/Commercial_Protocols/Illumina_truseq-dna-methylation-library-prep-guide-15066014-a.pdf) suggests that the libraries produced should end up with concentrations >3ng/μL, but we have plenty of DNA here to make a pool for running on the HiSeq2500.