Yesterday, I sheared the Lotterhos C.virginica gDNA in preparation for MBD enrichment. Today, I proceeded with MBD enrichment using the MethylMiner Kit (Invitrogen) on 12 of the 24 samples. I followed the manufacturer’s protocol for input DNA >1ug - 10ug with the following modifications:

• 2500ng of input DNA used

• discarded washes post-DNA-binding

• performed high-salt elution

• elutions were stored @ -20^oC until ready for EtOH precipitation next week

The full sample list and calculations are here (Google Sheet):

• Roberts_2017AdultExposureSampleInfo