Last week I performed MBD enrichment on 12 of the 24 Lotterhos C.viriginica mantle DNA on samples I had sheared on 20190306. I proceeded with MBD enrichment using the MethylMiner Kit (Invitrogen) on the 12 remaining samples of the 24. I followed the manufacturer’s protocol for input DNA >1ug - 10ug with the following modifications:

• 2500ng of input DNA used

• discarded washes post-DNA-binding

• performed high-salt elution

The full sample list and calculations are here (Google Sheet):

• Roberts_2017AdultExposureSampleInfo

Will proceed with ethanol precipitations of all 24 samples.