After a meeting last week, we realized we needed to update the paper-oly-mbdbs-gen GitHub repo with the most current versions of feature files we had.

As part of that, we needed a new intron GFF file generated. I also realized that the output from the MAKER annotation from 20190709 actually has 3’/5’ UTR features, so I decided to separate those out and create separate GFFs for them, as well.

The process was performed in the following Jupyter Notebook (GitHub):

• 20191214_olur_genome_feature_and_intron_splitting.ipynb

One thing to note in that Jupyter Notebook. The complementBed command threw an error related to sorting. Two things with this:

1. I don’t see an issue with the sorting.

2. It seems to have still run just fine and generated the expected output.

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RESULTS

Output folder:

• 20191214_olur_genome_feature_and_intron_splitting/

Here’s quick glance at IGV visualization of the intron BED file. Things look fine.

The files have been uploaded to the paper-oly-mbdbs-gen GitHub repo, as well as added to our Genomic Resources wiki.