Isolated RNA from the following hemolymph pellet samples:

• 6202_337_12

• 6142_474_26

• 6144_477_26

• 6122_489_26

• 6176_490_26

• 6136_491_26

• 6137_500_26

• 6125_503_26

• 6145_505_26

• 6131_507_26

• 6159_508_26

• 6206_509_26

• 6150_511_26

• 6161_512_26

• 6115_514_26

• 6179_515_26

• 6172_517_26

Isolated RNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

• Used 35uL from each RNAlater/hemocyte slurry.

• Mixed with equal volume of H₂O (35uL).

• Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.

• Performed on-column DNase step.

• RNA was eluted in 15uL H₂O

RNA was quantified on the Roberts Lab Qubit 3.0 using the RNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.

RNA was stored in the [-80_{o</sup>C freezer](http://b.link/srlab-80C) in Shellfish Box #8.}

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RESULTS

Qubit restuls (Google Sheet):

• 20200126_qubit_cbai_RNA

Sample ID	RNA
6202_337_12	23.7
6142_474_26	14.4
6144_477_26	20.3
6122_489_26	8.32
6176_490_26	10.7
6136_491_26	7.37
6137_500_26	13.8
6125_503_26	7.12
6145_505_26	4.7
6131_507_26	8.9
6159_508_26	7.74
6206_509_26	9.75
6150_511_26	4.12
6161_512_26	10.9
6115_514_26	5.96
6179_515_26	7.23
6172_517_26	Out of range