Since I was isolating gDNA from C.bairdi 6129_403_26 hemolymph, I figured I might as well co-isolate RNA since I was using the Quick DNA/RNA Microprep Plus Kit (ZymoResearch).
Followed the manufacturer’s protocol with the following notes/changes:
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Used 480uL of sample, combined with 1:1 H2O (480uL), with 4:1 Lysis Buffer (3840uL) and mixed in 15mL conical
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Flow-through was retained in a 15mL conical for RNA isolation
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Column began to clog during loading of the initial flow-through + EtOH mixture
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Eluted with 15uL
Quantified on the Roberts Lab Qubit 3.0 using the high-sensitivity RNA Assay (Invitrogen) and 1uL of sample.
RESULTS
Qubit results (Google Sheet):
[RNA] = 7.86ng/uL in 15uL
Yield = ~118ng
Yield is very, very low, considering the amount of starting material. Yield is likely due to clogging of column, due to overloading.
Sample was stored at -80oC in: