We received the RNAseq data from the RNA that was sent out by Grace on 20200212.

Sequencing is 150bp PE.

Grace has a Google Sheet that describes what the samples constitute (e.g. ambient/cold/warm, infected/uninfect, day, etc.)

• 02122020-samples-sent-to-genewiz

Genewiz report:

Project	Sample ID	Barcode Sequence	# Reads	Yield (Mbases)	Mean Quality Score	% Bases >= 30
30-343338329	72	ACTCGCTA+TCGACTAG	27,249,335	8,175	34.16	85.82
30-343338329	73	ACTCGCTA+TTCTAGCT	25,856,008	7,757	33.87	84.36
30-343338329	113	ACTCGCTA+CCTAGAGT	31,638,462	9,492	32.38	77.77
30-343338329	118	ACTCGCTA+GCGTAAGA	29,253,455	8,776	33.50	82.62
30-343338329	127	ACTCGCTA+CTATTAAG	27,552,329	8,266	33.14	81.13
30-343338329	132	ACTCGCTA+AAGGCTAT	27,518,702	8,256	34.86	88.87
30-343338329	151	ACTCGCTA+GAGCCTTA	33,430,314	10,029	35.01	89.35
30-343338329	173	ACTCGCTA+TTATGCGA	33,262,459	9,979	34.45	87.06
30-343338329	178	GGAGCTAC+TCGACTAG	29,495,389	8,849	35.01	89.62
30-343338329	221	GGAGCTAC+TTCTAGCT	25,902,415	7,771	34.76	88.40
30-343338329	222	GGAGCTAC+CCTAGAGT	53,808,137	16,142	30.90	71.11
30-343338329	254	GGAGCTAC+GCGTAAGA	16,771,613	5,031	35.14	90.03
30-343338329	272	GGAGCTAC+CTATTAAG	27,818,893	8,346	33.30	81.70
30-343338329	280	GGAGCTAC+AAGGCTAT	61,008,799	18,303	30.85	70.86
30-343338329	294	GGAGCTAC+GAGCCTTA	28,539,233	8,562	35.12	90.04
30-343338329	334	GGAGCTAC+TTATGCGA	25,916,895	7,775	34.98	89.39
30-343338329	349	GCGTAGTA+TCGACTAG	32,868,756	9,861	33.53	82.69
30-343338329	359	GCGTAGTA+TTCTAGCT	27,274,149	8,182	34.96	89.20
30-343338329	425	GCGTAGTA+CCTAGAGT	66,224,932	19,867	29.54	65.13
30-343338329	427	GCGTAGTA+GCGTAAGA	18,918,640	5,676	33.31	80.87
30-343338329	445	GCGTAGTA+CTATTAAG	30,745,388	9,224	33.07	80.83
30-343338329	463	GCGTAGTA+AAGGCTAT	19,531,145	5,859	34.27	86.08
30-343338329	481	GCGTAGTA+GAGCCTTA	50,592,084	15,178	31.92	75.59
30-343338329	485	GCGTAGTA+TTATGCGA	26,010,208	7,803	34.63	87.48

Confirmed that SFTP transfer from Genewiz to owl/nightingales/C_bairdi/ was successful:

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RESULTS

Output folder:

• owl/nightingales/C_bairdi/

Will update the nightingales Google Sheet with the appropriate info shortly.