Daily Bits - November 2022

20221121

  • Lab meeting

  • Short day due to early out for Audrey’s school.


20221118

  • Short day due to early out for Audrey’s school.

  • Meeting with Linda Rhodes

    • Re-ran Jupyter Notebook using her original Silva files, but set to 99% clustering.

    • Reviewed using Conda environments.

    • Introduced her to using git and GitHub - this repo


20221117

  • Pub-a-thon

  • Dealt with some power outages in the morning.


2022116

  • Went in to lab.

    • Showed Arianna around, got her keys, etc.

    • Updated/restarted Raven.

    • Worked on generating gene methylation coefficients of variation.

      • Struggled with converting data frame to matrix (required for rowcvs()).

      • RStudio Server locked up for a significant period of time.


20221115

  • Worked heavily on CEABIGR CoV gene methylation.

  • Worked heavily on CEABIGR diffs in max transcripts and mean gene methylation.


20221114

  • Lab Meeting.

    • Discussed Ch.7 of The Disordered Cosmos
  • C.virginica gene expression/DNA methylation meeting.


20221110

  • Sort of giving up on the Circos exploration - plots of the data we have don’t really fit into the Circos mode of data analysis (Circos is generally set up to examine spatial relationships of genome features).

  • Started trying to make dot plots of the differences between the maximum number of transcripts per gene.

20221108

  • Messed around a tiny bit with gettting max transcript count differences formatted for Circos.

20221107

  • Lab meeting.

  • C.virginica oyster gene expression meeting (i.e. CEABIGR)


20221104

  • Meeting with Linda Rhodes to discuss 16s results

  • Science Hour

  • Oyster meeting with Lotterhos group to dicuss CEABIGR data.

  • Power outage for a couple of hours…


20221103

  • Worked a bit on looking at Laura’s M.magister MBD-BSseq FastQC data (GitHub Issue).

  • Pub-a-thon.

  • Worked a bit on looking into Linda Rhodes 16s pipeline to see if we can figure out how to speed up the vsearch step. Nothing definitive found yet.


20221102

  • Finished updating the Ballgown Rmd script for CEABIGR repo.

    • Updated corresponding REAMDE(s) with file descriptions for output(s) from Rmd.
  • Possibly fixed owl/nightingales Synology Glacier backup. Existing Glacier backup was consistently failing immediately with unhelpful log message.

    • Created new backup task.

      • US West (Oregon).

      • New key/private key for Amazon AWS Glacier vault.

      • Task is running and has backed up ~265GB so far (out of 13.03TB). Will monitor progress over the next couple of weeks/months.

  • Worked on CEABIGR manuscript.


20221101

  • Worked on CEABIGR manuscript

  • Transferred and updated Ballgown Rmd script to CEABIGR repo.