Using cDNA from 20230721, performed qPCR on the following primer sets selected by Steven (GitHub Issue), along with Cg_Actin as a potential normalizing gene:
| SRID | Primer |
|---|---|
| 1170/1 | Cg_Actin |
| 1830/1 | Cg_DICER |
| 1172/3 | Cg_GAPDH |
| 1832/3 | Cg_IRF2 |
| 1834/5 | Cg_SACSIN |
| 1828/9 | Cg_VIPERIN |
| 1826/7 | Cg_cGAS |
| 1383/4 | Cg_citrate_synthase |
qPCR calcs:
- 20230726 - qPCR Calcs polyIC gigas cDNA (Google Sheet)
Reactions were run on white, low-profile, 96-well qPCR plates (USA Scientific). A total of two plates were run. One was run on a CFC Connect (BioRad) and the other on a CFX96 (BioRad). See the qPCR Report in the RESULTS section below for cycling params, plate layouts, etc.
All samples were run in singular. Normally duplicates/triplicates are run. However, due to time and sample constraints, samples were just run in singular. No template controls were run in duplicate.
RESULTS
Brief note(s) on results:
- Samples
T2andT15seem to come up earlier than all other samples in almost all of the primer sets.
qPCR Results file (CSV)
qPCR Data file (QPCRD - CFX Maestro required)
qPCR Report (PDF)
PLOTS
| SRID | Primer | Amplification Plot | Melt Curve |
|---|---|---|---|
| 1170/1 | Cg_Actin |  |
 |
| 1830/1 | Cg_DICER |  |
 |
| 1172/3 | Cg_GAPDH |  |
 |
| 1832/3 | Cg_IRF2 |  |
 |
| 1834/5 | Cg_SACSIN |  |
 |
| 1828/9 | Cg_VIPERIN |  |
 |
| 1826/7 | Cg_cGAS |  |
 |
| 1383/4 | Cg_citrate_synthase |  |
 |