After quantifying diploid MgCl₂ (Notebook) earlier today and proceeded to make cDNA. Reverse transcription was performed using oligo dT primers using M-MLV RT (Promega), per the manufacturer’s recommendations. Used 400ng of RNA in each reaction. I used 400ng (instead of the usual 100ng) to simplify pipetting for high concentration samples, without the need/time to dilute samples. All reactions were done on ice in 0.5uL PCR tubes.

cDNA was stored in the same -80^oC box as the original RNA.

• 202300816-cgig-polyIC-cDNA-calcs (Google Sheet)