Using diploid MgCl2 control cDNA from 20230816, performed qPCR on the following primer sets selected by Steven (GitHub Issue), along with Cg_Actin as a potential normalizing gene:
| SRID | Primer |
|---|---|
| 1170/1 | Cg_Actin |
| 1830/1 | Cg_DICER |
| 1172/3 | Cg_GAPDH |
| 1832/3 | Cg_IRF2 |
| 1834/5 | Cg_SACSIN |
| 1828/9 | Cg_VIPERIN |
| 1826/7 | Cg_cGAS |
| 1383/4 | Cg_citrate_synthase |
qPCR calcs:
- 20230817 - qPCR Calcs polyIC gigas cDNA (Google Sheet)
Reactions were run on white, low-profile, 96-well qPCR plates (USA Scientific). A total of two plates were run. One was run on a CFC Connect (BioRad) and the other on a CFX96 (BioRad). See the qPCR Report in the RESULTS section below for cycling params, plate layouts, etc.
Due to time and sample constraints, samples were just run in singular. No template controls were run in duplicate.
RESULTS
Brief note(s) on results:
-
Sample D14 comes up later than the other three samples in all of the primer sets.
-
Have not compared to the diploid poly:IC-inject qPCRs results (Notebook) yet.
qPCR Results file (CSV)
qPCR Data file (QPCRD - CFX Maestro required)
qPCR Report (PDF)
PLOTS
| SR ID | Primer | Amplification Plot | Melt Curve |
|---|---|---|---|
| 1830/1 | Cg_DICER |  |
 |
| 1172/3 | Cg_GAPDH |  |
 |
| 1832/3 | Cg_IRF2 |  |
 |
| 1834/5 | Cg_SACSIN |  |
 |
| 1828/9 | Cg_VIPERIN |  |
 |
| 1170/1 | Cg_actin |  |
 |
| 1826/7 | Cg_cGAS |  |
 |
| 1383/4 | Cg_citrate_synthase |  |
 |