FastQCFastQC Report
Tue 25 Mar 2014
BiGoRNA_CLCtrim_adp.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename BiGoRNA_CLCtrim_adp.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 47644404
Filtered Sequences 0
Sequence length 20-50
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 298887 0.6273286575271254 No Hit
GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 158528 0.3327316257330032 No Hit
ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151006 0.31694383248030555 No Hit
GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 141750 0.2975165771829153 No Hit
GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 134377 0.28204151740464634 No Hit
GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 127867 0.26837779311920873 No Hit
CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 124740 0.26181458792096546 No Hit
CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 105727 0.22190853725444862 No Hit
GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97328 0.20428002415561752 No Hit
CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90185 0.18928770732445305 No Hit
ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88279 0.1852872375106214 No Hit
CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82161 0.17244627511764024 No Hit
ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 79981 0.16787071153204058 No Hit
GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 76754 0.16109761809592582 No Hit
CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67752 0.1422034789227293 No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 67638 0.14196420633155574 No Hit
AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 58891 0.12360528216493168 No Hit
GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57640 0.12097958030915866 No Hit
CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56668 0.11893946663704723 No Hit
CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55559 0.1166118060790518 No Hit
CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55301 0.11607029442534322 No Hit
CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55049 0.11554137606590692 No Hit
CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 53992 0.11332285739160469 No Hit
CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 51868 0.10886483121921306 No Hit
ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51373 0.10782588444174891 No Hit
GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51236 0.10753833755586491 No Hit
CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50042 0.10503227199567865 No Hit
TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49839 0.10460619887279943 No Hit
CTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTCTTGAA 48739 0.10229742825621241 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19697400 3.836273 7.0163846 21
AAAAA 13733955 2.6724303 5.2335596 29
GGAGG 2855900 2.515796 8.472564 2
AGCAG 3983310 2.4660861 10.517376 21
CAGCA 3698720 2.3474848 6.277476 15
CCAGC 2454390 2.3293648 7.245107 40
GCTGC 2376265 2.2002883 7.2592516 12
CTTTT 7334885 2.1357899 5.495195 34
TTTCT 7334755 2.1357522 5.864383 37
CTTTC 4886025 2.1270883 5.8217964 4
GAAAG 4901265 2.029223 5.2090406 29
GCCCC 1404290 1.992936 6.892042 26
TGAAG 4789945 1.9834903 7.493846 45
TTTTC 6762960 1.9692553 5.561094 36
AGGTG 3242575 1.9585963 6.3233004 34
CCCCA 1964800 1.911611 5.213938 27
CAGCT 3011055 1.9113842 5.3195834 40
CTGCA 2983610 1.8939625 5.658643 13
GGCCA 2045085 1.8932945 9.387344 1
AGCTG 3010410 1.8640939 5.8756576 10
GGTGC 2006065 1.8119347 7.379024 5
CCCAC 1858490 1.8081787 9.684802 1
AGCAA 4258965 1.8076438 7.221312 11
GGGAG 2050470 1.8062829 8.810586 1
CTGGT 2899025 1.7954447 5.775868 1
AAAGG 4336325 1.7953264 5.117604 30
TAGCA 4166610 1.7687627 7.0304546 10
CCACA 2713610 1.765574 6.8326383 1
ACCAG 2702195 1.7150155 5.346091 39
GGGGA 1943920 1.7124218 15.695891 1
AAAAC 5851265 1.7025626 5.9843135 30
GCCTC 1788725 1.6979132 5.989176 15
GACAG 2664780 1.649778 6.200858 31
GCAGT 2650940 1.6415045 9.683783 22
CGCCC 1143015 1.6221405 6.378416 25
TTGCT 3807835 1.6170402 5.24353 41
ACAGC 2547100 1.6165806 5.137798 14
ACAGG 2600375 1.6099046 6.240147 32
AAATG 5569060 1.5809788 5.2632213 42
CTAAA 5426965 1.5793861 5.1007695 40
GAAGT 3693895 1.529622 7.049242 46
ACTTT 5249375 1.5282516 5.233288 33
AGGCC 1639025 1.5173728 7.2658896 32
CAGTT 3543270 1.5044194 7.1093173 23
ATGAA 5287190 1.5009596 5.3548965 44
GGTGA 2432305 1.4691732 6.1716275 35
CAGGT 2357570 1.4598451 6.1311235 33
GGGAC 1615100 1.458542 14.824455 2
ATCAA 4977935 1.448707 5.524612 11
GCCAT 2212715 1.4046069 5.6910176 2
GTGCT 2264785 1.4026427 5.371043 6
GCAAT 3282945 1.3936391 6.957583 12
AGTTT 4819240 1.3686066 6.238807 24
AAGGC 2170595 1.3438257 5.707393 31
AACTT 4607820 1.3412348 5.11817 32
AAACT 4559050 1.3268007 5.17426 31
GTTTA 4644945 1.3191088 6.102317 25
GAGGT 2151740 1.2997049 5.487677 3
AATCA 4398195 1.2799877 5.636413 10
AATGA 4337680 1.231407 5.190075 43
GGACT 1969475 1.2195302 10.874129 3
AAGCC 1796465 1.1401713 5.6111045 46
TCTAA 3825060 1.1133906 5.0956674 39
TTCTA 3781085 1.100788 5.0003834 38
CTAGC 1708055 1.0842544 9.569437 9
ATAGC 2512980 1.0667822 7.1181803 19
ACTAC 2110710 0.91854936 7.107994 5
CTACT 2075180 0.90324926 6.8711977 6
GAACG 1433975 0.8877808 5.3918023 38
ACTAG 1989560 0.8445858 6.419583 8
GACTA 1494690 0.6345091 6.8563495 4
Produced by FastQC (version 0.10.1)