FastQCFastQC Report
Wed 26 Mar 2014
BiGoRNA_GTGTCTAC.pe.qc.fq.pe.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename BiGoRNA_GTGTCTAC.pe.qc.fq.pe.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 48774044
Filtered Sequences 0
Sequence length 50
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGACACATCTCG 618782 1.2686706888606571 Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 528226 1.0830063629745363 Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 300280 0.6156553268373646 No Hit
GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 158798 0.3255789083226316 No Hit
ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151090 0.30977542071352543 No Hit
GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 142158 0.2914624016003266 No Hit
GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 134916 0.27661434020111186 No Hit
GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 128479 0.26341674682542215 No Hit
CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 124637 0.25553960627090916 No Hit
CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 105677 0.2166664712075136 No Hit
GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97286 0.199462648616957 No Hit
CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90055 0.18463714019694574 No Hit
ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88280 0.18099790946184408 No Hit
CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82055 0.16823497350352987 No Hit
ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 80005 0.16403191828834204 No Hit
GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 76666 0.1571860639646776 No Hit
CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67831 0.13907192112263647 No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 67751 0.1389078994557023 No Hit
AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 58963 0.12089011934298496 No Hit
GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57515 0.11792132717147669 No Hit
CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56766 0.11638567431480563 No Hit
CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55529 0.11384948928983621 No Hit
CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55304 0.11338817835158388 No Hit
CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55045 0.11285715820488454 No Hit
CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 54016 0.11074742951394394 No Hit
CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 51764 0.10613021958974736 No Hit
ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51517 0.10562380269308815 No Hit
GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51371 0.10532446315093331 No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG 50734 0.10401844062797008 Illumina Single End PCR Primer 1 (98% over 50bp)
CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50093 0.10270421702166013 No Hit
TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49949 0.10240897802117864 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19563570 3.9628701 7.2939725 21
AGCAG 4672780 2.6988955 20.057108 18
CAGCA 4387785 2.660571 20.79453 17
AAAAA 13662795 2.656524 5.256266 29
GGAGG 2855990 2.2223954 7.453902 2
CCAGC 2450250 2.2061307 6.8080764 40
CTTTT 7317800 2.1831126 5.6655164 34
TTTCT 7312910 2.1816537 6.024725 37
GAAAG 5559885 2.1626449 13.700252 23
CTTTC 4870750 2.1400566 5.952704 4
AAGAG 5356705 2.0836132 25.266804 7
GCTGC 2371090 2.0502539 6.7196045 12
AAAGA 7366665 2.0258987 9.575113 24
GGTGG 2580650 2.0246558 23.684233 40
TTTTC 6744530 2.0120893 5.7150884 36
GAAGA 5166385 2.009584 25.23724 6
GGAAG 3580230 1.969713 34.851505 5
TGAAG 4780045 1.8746012 6.984886 45
GCCCC 1400610 1.8725327 6.4911065 26
CAGGA 3227915 1.8643733 19.022709 20
CCCCA 1955970 1.8488504 5.061115 27
CAGCT 3004085 1.8365351 5.1405683 40
CTGCA 2978880 1.8211261 5.4270277 13
AGGTG 3240735 1.7976 5.840457 34
CGCCG 1403080 1.7867999 40.81793 46
GGCCA 2043925 1.75294 8.726096 1
CCCAC 1853865 1.7523372 9.356225 1
AGCTG 3005485 1.7501804 5.5584626 10
AGCAA 4246700 1.7341622 6.984663 11
CCACA 2709035 1.7244987 6.642519 1
TAGCA 4158970 1.7123063 6.856173 10
AGGAA 4399030 1.7111038 13.176795 21
AGACA 4179340 1.7066555 13.593879 39
CTGGT 2896440 1.7005534 5.5209403 1
AAAAC 5853805 1.6900666 5.971595 30
GCAGG 2063425 1.6856681 25.743029 19
GGAAA 4279380 1.6645632 12.92927 22
GGTGC 2009450 1.6550764 6.7265577 5
ACCAG 2700060 1.6372045 5.014004 39
GCCTC 1788650 1.6236923 5.7361174 15
AGAGC 2799700 1.6170455 36.442673 8
ATCTC 3703430 1.6138978 14.198161 45
TTTAA 8090970 1.6123054 5.0635557 26
GAGCG 1956915 1.5986571 50.601017 9
GGGAG 2050585 1.5956675 7.756995 1
TTGCT 3796530 1.5889033 5.1847763 41
CTAAA 5425195 1.5792046 5.080497 40
ACATC 3637510 1.572239 14.055556 43
CGGAA 2715015 1.5681331 36.189686 4
TCAGC 2534875 1.5496855 19.658281 16
ACTTT 5237110 1.5496361 5.357748 33
GCAGT 2652225 1.544467 9.202647 22
CGCCC 1151440 1.5394071 5.999777 25
AAATG 5551000 1.5391307 5.138518 42
GACAG 2663700 1.5384948 5.838352 31
CACAT 3554525 1.5363705 13.7536745 42
GGGGA 1950920 1.518113 13.899397 1
TGGTC 2570380 1.5091177 17.42043 42
AGACC 2483110 1.5056548 19.352533 31
ACAGG 2586365 1.4938278 5.8518114 32
TTCAG 3587240 1.4890647 13.918007 15
ACACA 3456640 1.4818734 13.873746 41
CCGAG 1720825 1.4758384 26.480108 28
AATGC 3566830 1.4685141 13.385968 24
AGGGA 2668355 1.4680322 17.635288 20
CAGTT 3531755 1.466033 7.015119 23
ATGAA 5272040 1.4617833 5.1486316 44
CTCGG 1681845 1.4542718 26.326874 37
GAAGT 3693620 1.4485354 6.5990176 46
ATCAA 4960850 1.4440397 5.5441737 11
TCGCC 1580545 1.4347796 26.982786 45
ATGCC 2342150 1.4318638 19.400152 25
TGCCG 1640325 1.4183699 26.752645 26
GCCGA 1644185 1.4101093 26.426357 27
CATCT 3225925 1.4058084 14.222633 44
AGGCC 1635610 1.4027551 6.7394023 32
CAGGT 2352860 1.370138 5.7856455 33
GTGGT 2444085 1.3668578 16.787022 41
TCTCG 2212545 1.3637558 19.189352 46
AGTTT 4805975 1.354568 6.2567677 24
AACTT 4602470 1.3507392 5.195414 32
GGTGA 2433125 1.3496275 5.7025766 35
GCAAT 3276775 1.3490942 6.8065114 12
GCCAT 2206175 1.348736 5.4985 2
GGGAA 2432475 1.3382597 17.471811 21
GTGCT 2263615 1.3290102 5.079741 6
GGGAC 1621395 1.3245617 13.522587 2
AAACT 4545740 1.3232065 5.2160134 31
GTTTA 4650040 1.3106176 6.1002088 25
GATCG 2226120 1.2963338 36.182964 1
ATCGG 2224930 1.2956408 36.176437 2
AATCA 4389030 1.2775903 5.6675096 10
TCGGA 2185135 1.2724669 36.41504 3
AAGGC 2165250 1.250601 5.366868 31
TAGAC 3031945 1.2482942 13.091565 38
GAGAC 2148710 1.2410479 18.278097 30
GACAC 2036075 1.2345915 19.034565 40
GTAGA 3102405 1.2166773 12.403288 37
GGTTC 2063045 1.211252 18.660313 13
AATGA 4329560 1.2004611 5.0080466 43
GAGGT 2155900 1.195854 5.050948 3
CCGGT 1369990 1.1846144 26.384502 34
CGGTG 1433085 1.1803554 23.871212 39
GTTCA 2805425 1.1645329 13.469884 14
GGACT 1967280 1.1456038 10.315313 3
GGAAT 2911945 1.1419841 12.77089 22
TGTAG 2877100 1.1375993 12.409763 17
GAATG 2898310 1.1366369 12.654592 23
TCTAA 3812900 1.1190151 5.137955 39
TTCTA 3773190 1.1164691 5.0887976 38
GGTCG 1341910 1.1052594 24.492006 43
AAGCC 1796435 1.0892836 5.290937 46
AGAGT 2767675 1.0854055 11.119669 26
AGCGG 1327320 1.0843239 25.798023 10
GACCG 1258935 1.0797058 26.230858 32
CGAGA 1868950 1.0794648 17.993305 29
AGCGT 1848235 1.0762805 18.515175 10
GTGTA 2718000 1.0746917 12.37387 16
GCGTC 1221450 1.0561736 26.737177 11
AGTGT 2656455 1.0503569 11.269964 28
GCGGT 1272905 1.0484238 25.728743 11
GATCT 2523375 1.047454 13.104827 34
CTAGC 1710215 1.045533 9.286482 9
ACCGG 1217540 1.044204 26.125538 33
ATAGC 2507125 1.0322186 6.9631467 19
GAGTG 1820580 1.0098557 15.425212 27
GGTAG 1816595 1.0076452 17.187832 36
CGTCG 1160185 1.0031985 26.927738 12
AGATC 2429470 1.0002469 12.999849 33
GTCGC 1155565 0.9992036 25.553751 44
GTCGT 1631060 0.95762557 18.3519 13
ACTAC 2112160 0.9129378 7.1410737 5
CTACT 2071930 0.90291524 6.9466968 6
TCGTG 1476450 0.8668511 18.12505 14
GTAGG 1538925 0.8536247 17.218767 18
CGTGT 1442710 0.8470418 17.994871 15
GAACG 1440545 0.8320272 5.0557775 38
ACTAG 1992755 0.8204452 6.279998 8
TAGGG 1474560 0.8179221 17.058483 19
CGGTT 1310665 0.7695157 18.24368 12
TCGGT 1244090 0.7304282 16.968712 38
CGGTA 1251650 0.7288718 17.770943 35
TAGAT 2534940 0.70864624 8.725278 32
GACTA 1497485 0.61653554 6.720068 4
Produced by FastQC (version 0.10.1)