FastQCFastQC Report
Tue 25 Feb 2014
s_1_sequence.txt

Summary

[OK] Basic Statistics

Measure Value
Filename s_1_sequence.txt
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 36020464
Filtered Sequences 0
Sequence length 36
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGGGTCTGCAGGCCTTTTTAACATGGCGCAGCGCCA 49433 0.1372358779165088 No Hit
AGGTGTTGCAGGGATGTGGGATTCCTATGTCCAACT 49118 0.13636137502282036 No Hit
AGTAGGTGCAGGGATGTGGGATTCCTATGTCCAACT 39639 0.1100457784219548 No Hit
AGCCATTGCAGGGATGTGGGATTCCTATGTCCAACT 38743 0.10755830352435217 No Hit
AGGGTCTGCAGGGGCTCTCTCCAAACAAAGCCTCTG 37789 0.10490980904632433 No Hit
AGTTAATGCAGGCCTTTTTAACATGGCGCAGCGCCA 36920 0.10249729153960925 No Hit
AGGGTCTGCAGGGATGTGGGATTCCTATGTCCAACT 36897 0.1024334389473717 No Hit
AGGGTCTGCAGGGCGAGTGGTGTGAAAAAGAATTTT 36634 0.10170329843613342 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GCAGG 24917725 26.485111 816.02954 8
TGCAG 25265410 23.741325 723.25494 7
TTGCA 10310755 8.565533 244.2837 6
CTGCA 6407445 7.6499825 192.23575 6
TCTGC 6344190 7.608089 187.9585 5
CAGGT 7951725 7.472054 213.73824 9
CAGGC 5496630 7.4231267 211.08397 9
CAGGA 7873695 7.3660293 216.01768 9
AGGTG 9246340 6.8383536 86.98888 1
ATGCA 7773200 6.4289484 176.87263 6
GTCTG 6464285 6.1013083 147.81206 4
GGGTC 5613975 5.9936 167.14986 2
GGTCT 6335505 5.9797597 147.62747 3
CAGGG 5391510 5.7306495 164.29807 9
GCGCA 3955615 5.342006 140.40604 2
AGCGC 3761160 5.0793967 140.15344 1
CATGC 4184675 4.996171 124.83562 5
AGGGT 6415910 4.745041 115.73339 1
GCCAT 3937455 4.7010098 114.53439 2
AGCCA 3934195 4.676357 113.71818 1
GGTGC 4214690 4.4996934 110.62849 5
CGCAT 3677350 4.3904653 124.09055 3
CCATT 4023210 4.2465234 100.92179 3
GTTGC 4356510 4.111887 111.194534 5
GTGCA 4346755 4.084546 97.66418 6
GCATG 4287420 4.0287905 97.87883 4
GGTGT 5190225 3.8555977 87.44235 2
AATGC 4429245 3.663277 90.702156 5
CTCTT 3434365 3.641087 86.006004 2
TTTGC 4109145 3.4287765 67.95197 5
CTTTG 4055185 3.3837507 68.05361 4
CATTG 4058175 3.371279 79.3705 4
ACTCT 3133180 3.3070912 85.53664 1
TGTTG 4904220 3.2207775 77.38254 4
ATTGC 3801875 3.1583605 79.451614 5
GTGTT 4802365 3.1538854 77.25867 3
TCTTT 3937005 2.9042828 60.08596 3
GTAGG 3781920 2.7970102 76.523094 2
AGTAG 4245900 2.76384 67.33766 1
GTTAA 4661030 2.6823177 63.209396 2
TAATG 4562265 2.6254811 63.03426 4
AGTTA 4535850 2.6102798 63.054733 1
TAGGT 3915865 2.5603225 67.58441 3
TTAAT 4812045 2.4481795 55.630825 3
TTTTT 4531950 2.3261962 8.141576 15
AGGAA 3426555 2.2206342 52.64578 10
AGGAC 2317745 2.1683056 58.24532 10
ATTTT 4168925 2.1304018 8.144691 32
AGGTA 3224060 2.09868 51.68081 10
AGGCT 2228660 2.0942206 49.102688 10
AGGTC 2211500 2.078096 38.05362 10
AGGCA 2211065 2.0685039 46.273857 10
AAAAA 4058770 2.037683 8.772891 25
AAACA 2709120 1.9633873 8.069817 23
AGGCC 1403330 1.8951786 43.34323 10
CCTTT 1719315 1.8228045 13.901103 13
GGTCA 1933935 1.8172746 16.123718 11
AAAAT 3528905 1.7795327 7.613041 26
AGGGC 1644230 1.747656 42.689766 10
TCAAA 2400255 1.7472653 7.1097603 23
AGGTT 2639905 1.7260574 34.06679 10
ACAAA 2346580 1.7006427 7.761878 25
AACAT 2323215 1.691184 9.856699 20
AATTT 3270530 1.6639173 7.3367815 31
CCAAC 1090795 1.6473726 21.606615 31
CAAAC 1551705 1.6233882 8.8137045 22
TCCAA 1522640 1.6000526 16.156076 30
GAAAA 2797575 1.5957412 9.898274 24
TTCCT 1500370 1.5906807 15.139999 22
CTTTT 2146090 1.5831456 10.358661 14
GCCTT 1307140 1.5675504 16.64037 12
AGGGA 2124920 1.5645897 35.401047 10
TTTTA 3035140 1.5510156 7.4318275 16
GGCTG 1445295 1.5430278 23.687214 11
CACTG 1285500 1.5347854 12.003346 27
CCTCC 695395 1.5227886 6.004677 28
CTCCA 1001410 1.5190934 12.025351 19
CTGCC 875680 1.5092337 11.8612795 25
GGAAA 2321210 1.5042976 23.145935 11
AGGAT 2302850 1.4990247 35.410053 10
CCAAA 1426425 1.4923205 9.067307 21
ACATG 1791120 1.481374 10.957013 21
CCAGT 1235070 1.4745759 12.617068 21
TGAAA 2555740 1.4642702 10.025908 23
GGCCT 1075060 1.4583001 23.03643 11
AACAA 1990850 1.4428337 7.1163673 24
CTCTC 936325 1.4266682 11.908106 17
CCTCT 935035 1.4247026 11.227086 31
GGACA 1512690 1.4151576 21.300442 11
TGTCC 1179155 1.4140681 15.111087 28
CATGG 1486770 1.3970836 12.174946 22
CAACT 1328250 1.3957796 14.467323 32
AACAC 1331845 1.3933713 8.714456 14
ACACC 912755 1.3784877 5.3913293 22
CAAAG 1673980 1.3783723 7.1423674 26
GCAGC 1008615 1.3621213 15.339824 28
TGCCT 1130750 1.3560197 12.112657 26
TAAAA 2678285 1.3505877 6.3017774 25
TTCTG 1583030 1.320921 6.9667606 23
ACCTT 1245535 1.31467 5.475245 16
TGCAC 1097555 1.3103939 11.989207 25
GTCCA 1093875 1.3060002 14.654813 29
TGGTC 1383070 1.3054091 9.679453 20
ATTCC 1235805 1.3044001 14.533415 21
GGCAA 1394060 1.3041763 12.712792 11
TCTCC 855415 1.3033864 12.452454 18
AGGGG 1556260 1.3019029 28.861774 10
AACTT 1767490 1.2923564 6.107547 29
TGCCA 1080260 1.289745 5.752289 32
TAACA 1763680 1.2838708 9.391311 19
GGTAT 1958505 1.2805356 21.250793 11
CAAGT 1545930 1.2785858 8.5897665 16
CACCT 841945 1.2771922 6.7435427 15
CACCC 575805 1.2553351 6.9780436 23
TCTAC 1184420 1.2501627 15.048903 30
CCCTC 568785 1.2455357 7.0286627 25
ACACT 1182125 1.2422254 8.435897 15
CTGTT 1474040 1.2299769 5.4746003 29
GTCAA 1485410 1.2285318 5.625104 12
GCTGC 894940 1.2139704 6.0667396 5
GCCTC 703255 1.2120594 12.471715 30
TTTAA 2381575 1.2116519 6.9030514 17
GGGAT 1630560 1.20592 18.84254 11
GGATG 1629725 1.2053025 13.119944 11
GCACT 1003005 1.1975088 11.732867 26
CCTAT 1134185 1.1971394 13.757929 24
GGCCA 880005 1.1884352 11.321464 11
GCCAG 876400 1.1835666 14.131393 20
CTACT 1117890 1.1799399 15.250552 31
AAGAA 2068190 1.1796988 5.7062573 28
ACTGT 1416880 1.1770555 5.035455 28
ACTTC 1114000 1.1758341 7.2002172 30
GATTC 1406995 1.1688436 11.474162 20
GACAA 1404165 1.1562037 8.492511 12
GGCAG 1087205 1.1555929 13.366454 11
CTCTG 957785 1.1485964 9.039559 32
TTGCC 954885 1.1451186 5.962109 31
TCTTG 1370465 1.1435512 5.085248 17
GAAAT 1993055 1.1418887 5.839845 12
GCAAG 1219425 1.1408012 9.679374 15
GGCAT 1208600 1.1356938 13.502879 11
CTGAT 1366620 1.1353027 9.001899 13
ATGTC 1358790 1.1287981 10.676451 27
AATAT 2226470 1.1277338 5.417122 26
GGTTT 1715870 1.1268735 10.6587515 11
AAATC 1547095 1.1262077 7.054814 27
GCTCT 936205 1.1227173 9.706107 14
GGCAC 830125 1.1210729 13.289145 11
AGGAG 1519830 1.1190588 25.203295 10
GGACT 1188470 1.1167781 23.322636 11
GCACA 937630 1.1145083 6.217213 12
CCCTG 646640 1.1144835 5.304159 17
CGCCA 649275 1.114079 18.060638 32
GGGCG 914100 1.1038898 23.117321 11
GGCTC 808810 1.0971365 10.693965 13
GTAAA 1889205 1.0823897 5.5281 24
TCCTA 1024455 1.0813187 13.878501 23
AAAGA 1888145 1.077001 5.410327 27
GTACA 1299950 1.0751442 8.190282 12
GAAAC 1296270 1.067362 9.265539 12
TGATC 1284735 1.0672777 9.727455 14
TGTGA 1631860 1.0669641 10.151609 21
GCTGA 1126415 1.0584664 13.579552 12
GGTGG 1255265 1.0547651 12.932024 11
CAGTA 1271340 1.051482 8.962641 22
TCTCT 989765 1.0493412 8.968089 16
GGTCC 772795 1.048283 11.638879 11
ATTCT 1413715 1.0382718 6.918995 22
GTGTG 1395575 1.0367132 11.33226 20
GGTAC 1100415 1.0340348 16.131384 11
GTGGT 1381495 1.0262538 12.3210945 17
TTAAC 1396580 1.0211537 8.520792 18
ACTGG 1084730 1.0192959 6.0244274 28
GGCGA 958945 1.019265 18.280966 12
ACCCT 671200 1.0181799 5.9419136 24
GACAC 851160 1.0117261 5.7482543 12
TATGT 1750270 1.0117128 7.5968637 26
AGGCG 950705 1.0105066 24.63735 10
TGATT 1744895 1.0086058 6.593281 20
GGCGC 656575 1.0074264 16.851103 25
ATATC 1366220 0.9989551 7.1936398 27
CTGGT 1058105 0.99869114 6.5642967 29
ATGGC 1061230 0.99721354 11.519023 23
AAAAG 1745645 0.9957187 5.3911605 26
GTGAA 1528325 0.9948528 10.592439 22
GGATT 1515505 0.9908875 9.744144 11
CACTA 937565 0.98523176 7.3446116 16
AAAGC 1194990 0.98396707 6.645855 27
ATGTG 1493810 0.9767026 10.330887 14
GATCT 1174240 0.9754853 5.0070696 15
GCGCC 499520 0.9738202 20.335281 31
GGGGC 798645 0.9644636 15.937585 11
TACTC 912335 0.9629754 15.016417 32
GATCA 1149045 0.9503358 5.1680803 21
TGGTG 1270655 0.94391555 11.881827 18
CTAGT 1129385 0.9382226 12.716704 14
GAACT 1125835 0.9311396 6.0602727 28
TATAA 1831215 0.92753243 5.8070526 13
CTGGC 683560 0.9272371 6.540666 2
GGTTC 963950 0.9098232 11.301676 11
GGACC 671675 0.9070883 9.631206 11
GTATA 1574475 0.90607494 9.37879 12
CGCAG 670345 0.90529203 15.501677 27
GAATT 1572940 0.90519154 5.0923047 30
GGGCA 845385 0.89856184 11.588897 11
GTGCC 661040 0.8966891 7.7600474 12
ATCTA 1220445 0.89236706 10.353666 29
AAGCC 749150 0.89047265 8.908825 28
GGTAA 1364690 0.88833576 12.371107 11
TATCT 1209260 0.8881143 6.168385 28
AGTAA 1549450 0.8877325 8.63419 23
GGCTT 930565 0.8783127 11.738685 11
GTGGG 1044450 0.8776229 9.720938 16
GATGT 1336205 0.87365514 9.85764 13
AATCT 1191510 0.8712102 6.6519837 28
GGAAC 926140 0.8664261 11.655202 11
GGAAG 1176045 0.8659281 12.485792 11
ACTAG 1041755 0.8615999 12.67782 13
GTAGC 915700 0.8604623 12.781753 17
CTTGG 911575 0.86038905 5.441337 18
AGAAT 1497385 0.85790265 5.307918 29
GGCCC 438640 0.8551339 7.271008 11
GGGCC 549965 0.8438476 8.5746565 11
GACTA 1011485 0.8365646 15.552894 12
GGAAT 1284710 0.83627343 11.16398 11
AAGTG 1280635 0.8336207 6.4112024 17
GGTTA 1274930 0.8335916 8.166373 11
CGAGT 882470 0.82923687 17.612675 14
GGTGA 1116635 0.82583433 9.622539 11
CAGCG 605335 0.817497 14.607576 29
AGAAC 988200 0.81369394 6.8052554 27
TGTGG 1089290 0.8091872 9.399281 15
AGCCT 677715 0.8091381 8.779135 29
AGTGG 1085340 0.8026894 11.825848 16
CGGCA 591020 0.7981648 8.716181 13
GAGTG 1077655 0.7970057 13.779489 15
CTATG 958725 0.796449 9.931358 25
GGCGG 657395 0.7938865 12.814598 11
TAGCC 662110 0.7905071 12.39493 18
GGATC 832425 0.7822107 7.444271 11
GGGGT 923725 0.77618104 10.057151 11
GTGAT 1184155 0.7742398 6.0419474 19
GGGTG 918045 0.7714083 6.3979883 12
GGGTA 1037495 0.7673044 8.913307 11
TAGTA 1320495 0.7599153 8.709082 15
GATGA 1165575 0.75872314 5.5366864 12
GGGCT 703500 0.75107163 8.89052 12
GGACG 697755 0.7416455 8.811942 11
GTATG 1124860 0.7354708 5.027777 12
GGTAG 981470 0.72586983 8.508996 11
GGCTA 771910 0.72534615 12.266202 11
AGTGA 1113960 0.7251247 5.9751515 18
TGGCG 676105 0.72182405 11.820718 24
GGGAC 677505 0.7201218 9.077564 11
GGATA 1093790 0.71199524 9.522261 11
GCGAG 666645 0.70857865 16.223673 13
GGTTG 951935 0.70715207 9.141492 11
GGAGG 830320 0.69461155 6.056123 11
GCGGC 431020 0.66134244 10.527213 12
GGAGA 876300 0.64522433 6.0836887 11
GGGAA 873810 0.6433909 5.49716 11
TGGGA 867880 0.64186156 9.370777 17
CTTCG 534730 0.64125973 7.3435593 31
TGGGT 857605 0.6370783 5.371374 15
GGGTT 846180 0.6285912 7.27818 11
GTGGC 581985 0.6213395 5.714016 12
TTCGA 747525 0.6209972 5.5164347 32
GGAGT 837710 0.6195486 9.284369 11
GGAGC 581760 0.6183542 7.295076 11
GGTCG 569075 0.60755664 5.064602 31
GCTAA 734470 0.6074551 6.0808187 12
GTCGC 434260 0.589066 5.8145003 32
GGGAG 694455 0.5809525 5.948382 11
GGCCG 367355 0.563657 5.555484 11
TTGCG 578750 0.5462526 6.665583 31
GACGA 564105 0.5277336 5.7541857 12
GGCGT 493650 0.5270313 5.2123556 11
TGCGA 548770 0.5156666 6.074601 32
Produced by FastQC (version 0.10.1)