In selecting qPCR targets from the Oly transcriptome (version 3) I came up with the following list. In general this was done by first focusing on stress response GO slim terms, followed by simple word searches. The table is available for download here The next step would be some joining & file converting to pull… Read more »
Monthly Archives:: May 2015
Stranded, sex, and arms still flailing
Normally I would not consider a week in review post, but so little progress was made (better than nothing) I thought I would give it a shot. Monday and Tuesday was in Oregon giving a seminar “Genomics on the Half Shell: Environmental Epigenetics, Open Science, and the Oyster“. (Yes, I will use that as an… Read more »
A short sequence on short sequence
A quick tutorial to check out primers on NCBI to see what the product size should be and how specific they are. Old school way is just take the primer with NNNs in the middle and blast. TATTCGTTGGAGACTTTATAACCCTNNNNNNNNNNNNNNNNNNNNNAAGGCTTAGAATTGCAAGGTCTATA You will get an output as such…. Scroll down to the alignments…. And look at the coordinates……. Read more »
Github Activity
- sr320 commented on issue RobertsLab/project-oyster-oa#18 October 15, 2017
- sr320 pushed to master at sr320/talk-ae-2017 October 13, 2017
- sr320 pushed to master at sr320/talk-ae-2017 October 12, 2017
- sr320 commented on issue RobertsLab/resources#98 October 12, 2017
- sr320 commented on issue RobertsLab/resources#97 October 12, 2017
- sr320 opened issue RobertsLab/resources#96 October 12, 2017
- sr320 commented on issue RobertsLab/project-oyster-oa#18 October 12, 2017
iPlant Activity
- DE: TopHat2-PE_analysis1 status changed. April 15, 2016
- DE: TopHat2-PE_analysis1 status changed. April 14, 2016
- DE: SAM_to_BAM_format_conversion_analysis1 status changed. March 22, 2016
- DE: Bowtie-2.2.1--Build-and-Map_analysis1 status changed. March 22, 2016
- DE: Bowtie-2.2.1_Bowtie2-Build_indexer_analysis1 status changed. March 21, 2016
Recent Comments