The first batch of sequencing came into today to verify sequence of Olympia oyster qPCR primers.
1) imported .ab1 files into CLC,
2) trimmed “CARM” sequences
Remove old trimming = Yes Quality trimming = Yes Quality limit = 0.05 Ambiguity trimming = Yes Ambiguity limit = 2 Vector trimming = No User vector trimming = No
3) aligned to comp7220_c0_seq2
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