Dirty and crude with Oly qPCR

Posted by & filed under Ostrea lurida.

Taking the most decent Ct values, I did the simple and crude calculation, normalizing with EF1 and looking at fold over minimum.

Screenshot_8_9_15__1_51_PM_1B77F561.png

Seems to be EF1 data is skewing. Will take a look with actin and also compare delta Ct, using this as a sound reference.

Comments are closed.