A few outliers remaining

Posted by & filed under Ostrea lurida.

Integrating last bit of qPCR data into master datasheet. This includes 5 runs post 8/15.

Resilience-Project_qPCR_data_at_master_·_jheare_Resilience-Project_1B91E568.png

likely error above – second EF1 was 18s test.

CARM
Looks nice, corrected…

Screenshot_8_29_15__6_14_AM_1B91E7B1.png

Elong factor
Correction is fine, but mechanical reps have some issues
Screenshot_8_29_15__6_28_AM_1B91EB21.png

As noticed in last batch of analysis these need to be checked at raw data level

Screenshot_8_29_15__6_32_AM_1B91EBFF.png

28s
Screenshot_8_29_15__6_40_AM_1B91EDBE.png

Thoughts on normalizing gene…
Noting that one EF1 rep was thrown out for mechanical (see above). Here is a crude look at EF1, actin, and 28s respectively…

Screenshot_8_29_15__6_46_AM_1B91EF43.png

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