In an attempt to determine most efficient means to get some high quality DNA from the archived oyster samples – a suite of samples were started out with M1 buffer and ProK.
The first ‘unit’ to go through will be the April Dabob samples. For today, I took 16 samples from the Hood canal population (code 3H13-16; yellow tubes). Samples were homogenized with plastic mortar and the other 8 did not get a mortar poke.
All samples were vortexed and placed at 37C at 2pm. This was done with air incubator, non-shaking.
Tomorrow, an additional set of samples will undergo a shorter Proteinase K digestion, subjected to Mollusc DNA extraction kit to try to determine best way to scale up.
Sam White
Remainder of extraction procedure can be found here: http://onsnetwork.org/kubu4/2015/09/16/dna-isolation-olympia-oyster-whole-body/