Monthly Archives:: September 2015

Using ESearch and EFetch to retrieve data

Posted by & filed under Tutorial.

Lets say there was this person, we will call her Emma for now, that needed to download lots of data but wanted to make it more robust and reliable. Here is a way to use NCBI ESearch and EFetch tools to do so. Complete documention at http://www.ncbi.nlm.nih.gov/books/NBK25498/. Specific example used is here Example one: Download… Read more »

Running the numbers

Posted by & filed under Ostrea lurida.

Today I brought another 16 samples into the DNA extraction pipeline. These were subjected to 30 minute ProK digestion at 60C as opposed to overnight. It became clear the number scheme was complicated thus I converted to 1-32. Will keep this running fwd and cross-referencing. In the future the number scheme will be simpler, but… Read more »

Upon improving extractions

Posted by & filed under Ostrea lurida.

In an attempt to determine most efficient means to get some high quality DNA from the archived oyster samples – a suite of samples were started out with M1 buffer and ProK. The first ‘unit’ to go through will be the April Dabob samples. For today, I took 16 samples from the Hood canal population… Read more »