We currently have a version (0.0.2) of the Ostrea lurida genome on CoGe. This is 38 scaffolds greater than 80k bp. Below is an effort to map gonad RNA-seq data to said genome.
Two male gonad and two female libraries were mapped to the genome using TopHat in Cyverse Discovery Environment.
Through the steps…
![CoGe__My_Data_1CEA6505.png](http://eagle.fish.washington.edu/cnidarian/skitch/CoGe__My_Data_1CEA6505.png)
![CoGe__My_Data_1CEA6557.png](http://eagle.fish.washington.edu/cnidarian/skitch/CoGe__My_Data_1CEA6557.png)
I moved the data in Discovery Environment to coge_data directory.
![CoGe__My_Data_1CEA6688.png](http://eagle.fish.washington.edu/cnidarian/skitch/CoGe__My_Data_1CEA6688.png)
Will see what Expression Analysis does…
![CoGe__My_Data_1CEA66F8.png](http://eagle.fish.washington.edu/cnidarian/skitch/CoGe__My_Data_1CEA66F8.png)
Some output
![CoGe__My_Data_1CEA6A27.png](http://eagle.fish.washington.edu/cnidarian/skitch/CoGe__My_Data_1CEA6A27.png)
This created two files and corresponding tracks: read depth and BAM alignment
![JBrowse_scaffold5824_3156__8360_1CEA6B71.png](http://eagle.fish.washington.edu/cnidarian/skitch/JBrowse_scaffold5824_3156__8360_1CEA6B71.png)
Will crank out other three libraries and soon will work on rough annotation.
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