Posts Categorized: Tutorial

Using ESearch and EFetch to retrieve data

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Lets say there was this person, we will call her Emma for now, that needed to download lots of data but wanted to make it more robust and reliable. Here is a way to use NCBI ESearch and EFetch tools to do so. Complete documention at http://www.ncbi.nlm.nih.gov/books/NBK25498/. Specific example used is here Example one: Download… Read more »

A short sequence on short sequence

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A quick tutorial to check out primers on NCBI to see what the product size should be and how specific they are. Old school way is just take the primer with NNNs in the middle and blast. TATTCGTTGGAGACTTTATAACCCTNNNNNNNNNNNNNNNNNNNNNAAGGCTTAGAATTGCAAGGTCTATA You will get an output as such…. Scroll down to the alignments…. And look at the coordinates……. Read more »