Comments for half-shell

Comment on Running the numbers by Agarose Gel – Olympia oyster Whole Body gDNA Integrity Check | Sam's Notebook

Thu, 17 Sep 2015 19:37:03 +0000

[…] be a distinct, high molecular weight band with no smearing if the gDNA was high quality and intact. These extractions also served as a comparison in slight differences in the extraction procedure (hom…. However, those differences seem to have no impact on the quality of the resulting […]

Comment on Running the numbers by DNA Isolation – Olympia oyster whole body | Sam's Notebook

DNA Isolation – Olympia oyster whole body | Sam's Notebook — Thu, 17 Sep 2015 17:41:37 +0000

[…] the extractions that Steven began yesterday and this morning using the E.Z.N.A. Mollusc DNA Kit (Omega Bio-Tek) after the RNase treatment @ […]

Comment on Upon improving extractions by kubu4

kubu4 — Thu, 17 Sep 2015 00:16:55 +0000

Remainder of extraction procedure can be found here: http://onsnetwork.org/kubu4/2015/09/16/dna-isolation-olympia-oyster-whole-body/

Comment on Upon improving extractions by DNA Isolation – Olympia oyster whole body | Sam's Notebook

DNA Isolation – Olympia oyster whole body | Sam's Notebook — Thu, 17 Sep 2015 00:16:46 +0000

[…] the extractions that Steven began yesterday using the E.Z.N.A. Mollusc DNA Kit (Omega Bio-Tek) after the RNase treatment @ […]

Comment on There is something about TEs by Steven Roberts

Steven Roberts — Thu, 09 Jul 2015 22:29:56 +0000

fu- the direction of methylation in TEs in predominantly hypo, or loss of methylation.

Comment on A closer look at DMRs by Heat Stress DDMRs | half-shell

Heat Stress DDMRs | half-shell — Tue, 07 Jul 2015 22:44:06 +0000

[…] a summary of 10 Dramatically Differentially Methylated Regions (DDMRs), that is, those covered in this post that are found across a majority of the oysters exposed to acute heat […]

Comment on Side track with tracks by First steps at an aggregated view of all DNA methylation data (updated) | half-shell

Fri, 26 Jun 2015 18:19:23 +0000

[…] Side track with tracks June 26, 2015 […]

Comment on Re-defining Cgigas Canonical features by Steven Roberts

Steven Roberts — Fri, 27 Feb 2015 18:37:26 +0000

IGV track
`/Volumes/web/halfshell/qdod3/igv-session/Ensembl-v9-tracks.xml`

Comment on Intersecting DMRs by Steven Roberts

Steven Roberts — Fri, 27 Feb 2015 18:31:37 +0000

Next step will be to determine stats on this, but then move onto more intersects including

gene by function (GOslim)
new gene IDs defined by Tophat analysis
differentially expression genes

Comment on Differential Methylation in the Kitchen by Intersecting DMRs | half-shell

Intersecting DMRs | half-shell — Fri, 27 Feb 2015 18:24:48 +0000

[…] try to determine where the DMRs defined by three *sig.bedGraph files described here are located on genome – intersectbed was used, some steps and […]