In order to find putative NonSyn SNPS-

1) v2 was generated which limits contigs to evalue <1E-20 and all sequences were oriented 5'-3'

2) from this data set, putative ORF were identified based on sequences between stop codon (min 200 bp)

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New analysis

Will blast against SP

genefish:bin Steven$ ./blastx -query /Users/Shared/data/blast/query/QPX_transcriptome_v2orf.fasta -db /Users/Shared/data/blast/db/uniprot_sprot -num_threads 8 -out /Users/Shared/data/blast/out/QPXv2_1_blastx_SwissProt.txt -outfmt 6 -max_target_seqs 1