Input parameters: --infile T1_transcripts.gtf,T2_transcripts.gtf,U1_transcripts.gtf,U2_transcripts.gtf --min-isoform-fraction 0.1 --user_annotation Crassostrea_gigas.GCA_000297895.1.21.gtf --user_database Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel.fa --version 2.1.1 -p 6 Executing: cuffmerge -o cuffmerge_out -g Crassostrea_gigas.GCA_000297895.1.21.gtf -s Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel.fa --min-isoform-fraction 0.1 gtf_to_merge.txt [Thu Feb 6 09:55:19 2014] Beginning transcriptome assembly merge ------------------------------------------- [Thu Feb 6 09:55:19 2014] Preparing output location cuffmerge_out/ [Thu Feb 6 09:55:23 2014] Converting GTF files to SAM gtf_to_sam: /lib64/libz.so.1: no version information available (required by gtf_to_sam) [09:55:23] Loading reference annotation. gtf_to_sam: /lib64/libz.so.1: no version information available (required by gtf_to_sam) [09:55:26] Loading reference annotation. gtf_to_sam: /lib64/libz.so.1: no version information available (required by gtf_to_sam) [09:55:28] Loading reference annotation. gtf_to_sam: /lib64/libz.so.1: no version information available (required by gtf_to_sam) [09:55:32] Loading reference annotation. [Thu Feb 6 09:55:36 2014] Quantitating transcripts cufflinks: /lib64/libz.so.1: no version information available (required by cufflinks) You are using Cufflinks v2.1.1, which is the most recent release. Command line: cufflinks -o cuffmerge_out/ -F 0.1 -g Crassostrea_gigas.GCA_000297895.1.21.gtf -q --overhang-tolerance 200 --library-type=transfrags -A 0.0 --min-frags-per-transfrag 0 --no-5-extend -p 1 cuffmerge_out/tmp/mergeSam_fileWErbbR [bam_header_read] EOF marker is absent. [bam_header_read] invalid BAM binary header (this is not a BAM file). File cuffmerge_out/tmp/mergeSam_fileWErbbR doesn't appear to be a valid BAM file, trying SAM... [09:55:36] Loading reference annotation. [09:55:39] Inspecting reads and determining fragment length distribution. Processed 38485 loci. > Map Properties: > Normalized Map Mass: 182896.00 > Raw Map Mass: 182896.00 > Fragment Length Distribution: Truncated Gaussian (default) > Default Mean: 200 > Default Std Dev: 80 [09:55:41] Assembling transcripts and estimating abundances. Processed 38485 loci. [Thu Feb 6 10:00:57 2014] Comparing against reference file Crassostrea_gigas.GCA_000297895.1.21.gtf You are using Cufflinks v2.1.1, which is the most recent release. No fasta index found for Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel.fa. Rebuilding, please wait.. Fasta index rebuilt. [Thu Feb 6 10:01:38 2014] Comparing against reference file Crassostrea_gigas.GCA_000297895.1.21.gtf You are using Cufflinks v2.1.1, which is the most recent release.