[2014-02-05 18:18:28] Beginning TopHat run (v2.0.9) ----------------------------------------------- [2014-02-05 18:18:28] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-02-05 18:18:28] Checking for Samtools Samtools version: 0.1.18.0 [2014-02-05 18:18:28] Checking for Bowtie index files (genome).. [2014-02-05 18:18:28] Checking for reference FASTA file [2014-02-05 18:18:28] Generating SAM header for Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel format: fastq quality scale: phred33 (default) [2014-02-05 18:18:52] Reading known junctions from GTF file [2014-02-05 18:18:56] Preparing reads left reads: min. length=49, max. length=49, 12520524 kept reads (482 discarded) [2014-02-05 18:26:32] Building transcriptome data files.. [2014-02-05 18:26:38] Building Bowtie index from Crassostrea_gigas.GCA_000297895.1.21.fa [2014-02-05 18:27:43] Mapping left_kept_reads to transcriptome Crassostrea_gigas.GCA_000297895.1.21 with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 18:31:40] Resuming TopHat pipeline with unmapped reads [2014-02-05 18:31:40] Mapping left_kept_reads.m2g_um to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) [2014-02-05 18:35:25] Mapping left_kept_reads.m2g_um_seg1 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 18:36:36] Mapping left_kept_reads.m2g_um_seg2 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 18:38:10] Searching for junctions via segment mapping [2014-02-05 18:53:56] Retrieving sequences for splices [2014-02-05 18:54:29] Indexing splices [2014-02-05 18:57:59] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 18:59:51] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 19:01:59] Joining segment hits [2014-02-05 19:02:45] Reporting output tracks ----------------------------------------------- [2014-02-05 19:08:03] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2014-02-05 19:08:03] Run complete: 00:49:34 elapsed