[2014-02-05 19:08:14] Beginning TopHat run (v2.0.9) ----------------------------------------------- [2014-02-05 19:08:14] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-02-05 19:08:14] Checking for Samtools Samtools version: 0.1.18.0 [2014-02-05 19:08:14] Checking for Bowtie index files (genome).. [2014-02-05 19:08:14] Checking for reference FASTA file [2014-02-05 19:08:14] Generating SAM header for Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel format: fastq quality scale: phred33 (default) [2014-02-05 19:08:15] Reading known junctions from GTF file [2014-02-05 19:08:17] Preparing reads left reads: min. length=49, max. length=49, 24245050 kept reads (358 discarded) [2014-02-05 19:13:42] Building transcriptome data files.. [2014-02-05 19:13:46] Building Bowtie index from Crassostrea_gigas.GCA_000297895.1.21.fa [2014-02-05 19:14:31] Mapping left_kept_reads to transcriptome Crassostrea_gigas.GCA_000297895.1.21 with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 19:20:29] Resuming TopHat pipeline with unmapped reads [2014-02-05 19:20:29] Mapping left_kept_reads.m2g_um to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) [2014-02-05 19:27:15] Mapping left_kept_reads.m2g_um_seg1 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 19:29:19] Mapping left_kept_reads.m2g_um_seg2 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 19:32:28] Searching for junctions via segment mapping [2014-02-05 21:10:58] Retrieving sequences for splices [2014-02-05 21:11:55] Indexing splices [2014-02-05 21:24:13] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 21:29:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 21:36:29] Joining segment hits [2014-02-05 21:38:11] Reporting output tracks ----------------------------------------------- [2014-02-05 21:47:56] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2014-02-05 21:47:56] Run complete: 02:39:42 elapsed